Metabolomics Platform

The mass spectrometers used in the metabolomics platform enable the measurement of all potential classes of metabolites, for both targeted and untargeted approaches. Profiling is performed using a high-resolution mass spectrometer (HRMS) coupled with ultra-high-performance chromatography (UHPLC). Targeted metabolomic 
analysis relies on the MRM (multiple reaction monitoring) technique following separation by UHPLC, depending on the properties of the metabolite of interest. We routinely quantify amino acids and their derivatives, fatty acids, acylcarnitines, acylglycerides, nucleotides, neutral lipids, and molecules involved in glycolysis and the Krebs cycle, among others.
Analyses can be performed on various types of biological sample extracts, such as biofluids, fecal samples, and tissues from human cohorts, animal experiments, or cell cultures.
Mass spectrometry imaging technology enables unbiased analysis of the spatial distribution of thousands of metabolites within biological tissues and cell cultures.
The platform brings together all the technical resources required for a metabolomics project, from the extraction of metabolites from the biological matrix to data analysis and the reporting of results. It is open to researchers at Gustave Roussy, as well as to external laboratories, whether public or private.

KROEMER Guido - Platform Scientific Director
Other status (including apprentice)
Civil servant researcher 
Employer: Other

APRAHAMIAN Fanny
Research Technician or equivalent 
Employer: Gustave Roussy

GRAJEDA IGLESIAS Claudia - Platform Operational Manager
Research Engineer or equivalent 
Employer: Gustave Roussy